Nipah F DMS

Visualizing Deep Mutational Scanning Data

Overview

This section hosts different visualizations of deep mutational scanning data from the Nipah virus F project. Explore the data through interactive plots and figures that illustrate the breadth of mutational effects on the Nipah virus F protein. Code for Vue and D3 used to generate the visualizations are shown below the figures.

Cell Entry

Static Cell Entry Heatmap

Focus on specific sites or change the visualization parameters.

Effects of Nipah F Mutations on Cell Entry

Heatmap is zoomable and draggable inside container.

Effects of Nipah F Mutations on Cell Entry in Windows

Explore cell entry data in smaller windows

Distribution of Effects by Amino Acid Mutation

Animation showing how mutation effects vary by amino acid

Antibody Escape

Antibody Escape Heatmaps

Switch between different antibodies to see how mutations affect neutralization.

Antibody Escape Interactive Heatmap

Explore antibody escape data in twenty-residue windows

Antibody Escape Animation

Animation showing escape aggregated by site for the different antibodies.

Antibody Escape Line Plot

Aggregate antibody escape data across all antibodies. Hover over lines to see details.

Antibody Escape

Toggle between antibody escape datasets aggregated by site.

Antibody Escape and Cell Entry Dot Plot

Toggle between phenotypes

Antibody Escape Analysis for Hendra Virus

Which mutations in Hendra virus affect antibody neutralization?

Antibody Escape Dot Plot

Hover over points to get more information.

Antibody Escape Logo Plots

Top escape mutations for each antibody colored by effect on cell entry

RBP and F Comparisons

Correlations of Effects of Mutations with Bat Ephrin-B2 vs -B3

Difference in effects of mutations for RBP and F

Differences in Mutational Tolerance Between RBP and F

Relative frequencies of mutation effects for RBP and F proteins

Other

Escape and Entry Slideable Heatmaps

Antibody escape and cell entry effects in one combined figure

Lentivirus Genome

Schematic of lentivirus genome used for deep mutational scanning

Animation of Cell Entry by Amino Acid Letters

Unmutated residue and site number are placed near the top, with increasing deleterious effects as you go down