Deep mutational scanning using HCoV-229E Spike strain 1984
Analysis by Harari at al (2026)
See https://github.com/dms-vep/229E_spike_1984_DMS for full code.
Documentation of results rendered as of Tue Feb 24 23:30:04 2026
Barcode to codon-variant lookup table
Analysis notebooks
Data files
Count barcodes for variants
Analysis notebooks
Data files
Barcode count CSVs (click triangle to expand/collapse)
Functional effects of mutations
Final summary plots
Interactive plots of average mutation functional effects (click triangle to expand/collapse)
Interactive plots of average mutation functional effects from single-mutant variants only (click triangle to expand/collapse)
Analysis notebooks
- Analysis of functional scores
Notebooks with per-selection global epistasis fitting (click triangle to expand/collapse)
Notebooks averaging mutation functional effects (click triangle to expand/collapse)
Data files
Per-variant functional score CSVs (click triangle to expand/collapse)
Per-selection mutation functional effect CSVs (click triangle to expand/collapse)
Average mutation functional effects (CSV files) (click triangle to expand/collapse)
Average mutation functional effects from single-mutant variants only (CSV files) (click triangle to expand/collapse)
Average mutation latent-phenotype effects (CSV files) (click triangle to expand/collapse)
serum escape
Final summary plots
antibody escape mutation effect plots (click triangle to expand/collapse)
Analysis notebooks
Fits of polyclonal models to individual antibody escape selections (click triangle to expand/collapse)
Average selections for antibody escape (click triangle to expand/collapse)
Data files
Probability (fraction) escape for each variant in each antibody escape selection (CSVs) (click triangle to expand/collapse)
antibody escape CSVs (click triangle to expand/collapse)
APN affinity
Final summary plots
receptor affinity mutation effect plots (click triangle to expand/collapse)
Analysis notebooks
Fits of polyclonal models to individual receptor affinity selections (click triangle to expand/collapse)
Average selections for receptor affinity (click triangle to expand/collapse)
Data files
Probability (fraction) escape for each variant in each receptor affinity selection (CSVs) (click triangle to expand/collapse)
receptor affinity CSVs (click triangle to expand/collapse)
Integrated summary plots
cell entry and binding
summary of all sera
summary of all phenotypes
dms-viz visualizations
dms-viz JSON files
Notebooks prepping dms-viz JSONs
binding vs escape effect
Row-wrapped heatmaps