Pseudovirus deep mutational scanning of the CHIKV envelope proteins (181/25 strain)
Analysis by Ju et al (2025)
See https://github.com/dms-vep/CHIKV-181-25-E-DMS for full code.
Documentation of results rendered as of Fri Nov 7 06:49:22 2025
Barcode to codon-variant lookup table
Analysis notebooks
Data files
Count barcodes for variants
Analysis notebooks
Data files
Barcode count CSVs (click triangle to expand/collapse)
Functional effects of mutations
Final summary plots
Interactive plots of average mutation functional effects (click triangle to expand/collapse)
Interactive plots of average mutation functional effects from single-mutant variants only (click triangle to expand/collapse)
Interactive plots of differences in functional effects between conditions (click triangle to expand/collapse)
Analysis notebooks
- Analysis of functional scores
Notebooks with per-selection global epistasis fitting (click triangle to expand/collapse)
Notebooks averaging mutation functional effects (click triangle to expand/collapse)
Notebooks taking differences in functional effects between conditions (click triangle to expand/collapse)
Data files
Per-variant functional score CSVs (click triangle to expand/collapse)
Per-selection mutation functional effect CSVs (click triangle to expand/collapse)
Average mutation functional effects (CSV files) (click triangle to expand/collapse)
Average mutation functional effects from single-mutant variants only (CSV files) (click triangle to expand/collapse)
Average mutation latent-phenotype effects (CSV files) (click triangle to expand/collapse)
Differences in functional effects (CSV files) (click triangle to expand/collapse)
Mxra8 binding
Final summary plots
receptor affinity mutation effect plots (click triangle to expand/collapse)
Analysis notebooks
Fits of polyclonal models to individual receptor affinity selections (click triangle to expand/collapse)
Average selections for receptor affinity (click triangle to expand/collapse)
Data files
Probability (fraction) escape for each variant in each receptor affinity selection (CSVs) (click triangle to expand/collapse)
receptor affinity CSVs (click triangle to expand/collapse)
Integrated summary plots
entry 293T-Mxra8 C636 293T-TIM1 Mxra8-binding
binding mouse vs human Mxra8
Distances to Mxra8 in structures
Distances to Mxra8
Compare binding to human vs mouse Mxra8
Charts and notebook
Compare Mxra8 binding to cell entry
Charts and notebooks
Compare entry among cells
Final plots
Analysis notebooks
Data files
dms-viz visualizations
dms-viz JSON files
Notebooks prepping dms-viz JSONs
Row-wrapped heatmaps
Heatmap HTMLs
additional data CSVs
CSVs