Data and interactive figures for pseudovirus deep mutational scanning of influenza H7 HA
This website provides interactive visualizations and raw data about the effects of mutations to the hemagglutinin protein of the H7 influenza strain A/Anhui/1/2013. See Ahn, Yu, et al (2026) for study details.
Because the dataset is so rich, the intereactive visualizations here are generally a better way to explore the data then the static figures in the paper. Specifically, see the Cell Entry page for measurements of how H7 HA mutations affect its ability to mediate entry into cells expressing different sialic acid linkages.
Note that throughout this website we use H3 numbering of the HA protein (see here).
The measurements shown on this site were made using pseudovirus deep mutational scanning with lentiviral particles pseudotyped with hemagglutinin. These pseudoviruses do not encode any viral proteins other than hemagglutinin, and so are only able to undergo a single round of cell entry and are not fully replicative agents capable of spreading or causing disease. They therefore provide a safe way to study mutations to viral entry proteins at biosafety-level-2.
Links to the deep mutational scanning measurements in numerical form (eg, as CSVs) are included on the Cell Entry page.
For more detailed documentation of the analysis (including additional interactive plots and links to CSVs) as automatically generated by dms-vep-pipeline-3, see the Appendix.
The full computer code, processed data, and analysis pipeline is available on GitHub at https://github.com/dms-vep/Flu_H7_Anhui13_DMS.
To see the less fancy documentation automatically build by the pipeline, see the Appendix.