Cell entry
Effects of mutations on cell entry
Deep mutational scanning of rabies G (Pasteur strain)
Pseudovirus deep mutational scanning to measure how rabies G mutations affect cell entry and escape from a panel of monoclonal antibodies
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Summary
This website provides easy access to interactive visualizations of the results of deep mutational scanning of rabies G. The links in the boxes above take you to pages that access the data in different forms.
The study that generated these data was performed in the Bloom lab; please see Aditham et al (2024) for full details.
In all the visualizations and data, protein sites are numbered in the standard scheme starting with 1 assigned to the first residue of G's ectodomain (rather than the N-terminal methionine).
The experiments were performed using pseudovirus deep mutational scanning, which enables the safe study of mutants of viral entry proteins using single-cycle replicative pseudoviruses that are not human pathogens.
The GitHub repository with the full analysis and processed data is at https://github.com/dms-vep/RABV_Pasteur_G_DMS. This analysis used dms-vep-pipeline-3, and the full output of the pipeline (which represents less user-friendly but more extensive documentation) is in the Appendix.