Pseudovirus deep mutational scanning of HCoV-229E spike protein (1984 strain)

Effects of HCoV-229E spike mutations on human APN binding. ​

Interactive plot ​

The plots below show how mutations affect the binding of the spike to its human APN receptor. These plots are interactive, and allow you to zoom and mouseover sites and mutations.

  • The zoom bar at the top of the plot shows different regions of spike, and can be used to zoom in on specific sites.
  • The line plot summarizes the effects of mutations on receptor binding at each site (more negative values indicate impaired binding). The binding at a site is quantified using the site summary statistic specified by the interactive option at the bottom of the plot (e.g., mean effect of mutations at a site).
  • The heatmap shows how each individual mutation affects binding. The X's indicate the amino-acid identity in the 1984 strain. Light gray tiles indicate mutations that were missing (not measured) in the library. Dark gray indicates mutations that are too deleterious for cell entry to measure their effect on receptor binding.

Click on the expansion box in the upper right of the plot to enlarge for easier viewing, or click here to open the plot in a stand-alone window.

For a wrapped version of the heatmap, click here.

Numerical values ​

The pre-filtered numerical data plotted on this page can be found here. Alternatively, the unfiltered data are available here. Note the unfiltered data have not been filtered for QC criteria like times_seen, so either make sure you understand the filters in the file or we recommend just using the pre-filtered data.

Interactive structures showing hAPN binding effects ​

Below are interactive visualizations showing deep mutational scanning data for hAPN binding in the context of the full spike protein structure. These visualizations were generated using the dms-viz platform. Use Protein Options on the left to set Selection representation to the desired graphics. Results are shown for the spike trimer with all RBDs in the “down” conformation (PDB 6U7H) and for a spike structure with one RBD in the “up” conformation, including the hAPN receptor (pdb 8WDE).

PDB ID 6U7H ​

For a standalone version of this visualization, use this link.

PDB ID 8WDE ​

For a standalone version of this visualization, use this link.