Evolution-resistant and evolution-sensitive sera escape
We measured how mutations in the HCoV-229E spike affect neutralization by evolution-resistant compared to evolution-sensitive sera. All sera samples were isolated in the 1980-1990's.
Below are several ways to explore these data:
Line plots and heatmaps showing effects of mutations summarized by sera group
The plot below is interactive, and allows you to zoom and mouseover sites and mutations. Click on the expansion box in the upper right of the plot to enlarge it for easier viewing, or click here for a standalone version of the plot. The line plots summarize the effects of mutations at each measured site, while the heatmaps below show the effects of individual mutations. Each panel groups all evolution-resistant or evolution-sesitive sera. Note that the two different shades of gray in the heatmaps have differing meanings: light gray means a mutation was missing (not measured) in the library, whereas dark gray means a mutation was measured but was so deleterious for cell entry it is not possible to reliably estimate its effect on other phenotypes (the threshold for how deleterious a mutation must be for cell entry to be shown in dark gray is controlled by the cell entry slider at the bottom of the plot). This plot also includes the cell entry and hAPN binding heatmaps for easy comparison of the mutations’ effects on different phenotypes.
Per-individual comparison of effects of mutations neutralization by evolution-resistant and evolution-sensitive serum
The plots below show the effects of mutations on neutralization by sera from the same individual. The scatter plots show the effects of individual mutations, and the line plots show the total effects of mutations at each site. The zoom bar at top allows you to examine just certain regions of spike.
Evolution-sensitive sera
SD85_3 serum
SD85_7 serum
SD87_2 serum
SD88_4 serum
SD93_4 serum
Eevolution-resistant sera
SD86_11 serum
SD88_10 serum
SD95_3 serum
Interactive structures displying sera groups neutralization
Below are interactive visualizations showing deep mutational scanning data for human sera escape in the context of the full spike protein structure. These visualizations were generated using the dms-viz platform. Use Protein Options on the left to set Selection representation to the desired graphics. Results are shown for the spike trimer with all RBDs in the “down” conformation (PDB 6U7H) and for a spike structure with one RBD in the “up” conformation, including the hAPN receptor (PDB 8WDE).
Total escape of evolution-resistant sera using PDB 6U7H
For a standalone version of this visualization, use this link.
Total escape of evolution-resistant sera using PDB 8WDE
For a standalone version of this visualization, use this link.
Total escape of evolution-sensitive sera using PDB 6U7H
For a standalone version of this visualization, use this link.
Total escape of evolution-sensitive sera using PDB 8WDE
For a standalone version of this visualization, use this link.