Pseudovirus deep mutational scanning of CHIKV envelope proteins

Visualizations of deep mutational scanning on envelope protein structures ​

This provides access to the effects of mutations mapped onto three-dimensional structures of the envelope proteins, as rendered by dms-viz.

It uses two structures, both from this paper, that are cryo-EM structures of the CHIKV envelope proteins bound to mouse Mxra8. In both structures, there are four envelope heteromers (E2 / E1 or E3 / E2 / E1) in the asymmetric unit even though envelop actually forms a trimer. Note that as described in the paper, Mxra8 contacts E2 / E1 in three different modes: wrapped, intraspike, and interspike. The structures are:

  • PDB ID 6nk7: E3 / E2 / E1 on CHIKV bound to one copy of mouse Mxra8
  • PDB ID 6nk6: E2 / E1 on CHIKV virus-like particles bound to four copies of mouse Mxra8.

Note that all of the dms-viz visualizations are interactive, and allow you to click on sites in the line plot to show them on the envelope proteins structure, as well as various other interactive options. In all of the structures below, the envelope proteins are initially gray while Mxra8 is shown in yellow; clicking on a site in the line plot will color it on the envelope protein structure based on its measured effect:

Effects of mutations on cell entry ​

Below is a visualization of the effects of mutations on cell entry on the trimer in 6nk7. Click on the Sidebar and Chart Options to select which cell line to show entry for; click on the line plot to highlight sites on the structure. To view it in a standalone page (which may be easier to interact with), click here.

For additional visualizations, see:

Differences in effects of mutations on entry in different cells ​

Below is a visualization of the differences in effects of mutations on entry in two different cell lines on the trimer in 6nk7. Click on the Sidebar and Chart Options to select which cell pair to show the differences for; click on the line plot to highlight sites on the structure. To view it in a standalone page (which may be easier to interact with), click here.

For additional visualizations, see:

Effects of mutations on Mxra8 binding ​

Below is a visualization of the effects of mutations on Mxra8 binding on the trimer in 6nk7. Click on the Sidebar and Chart Options tho select whether to show binding to mouse Mxra8 or human Mxra8. To view it on a standalone page (which may be easier to interact with), click here.

For additional visualizations, see: