Experimental measurements of how mutations to the F protein from RSV (subtype A Long strain) affect its cell entry function and neutralization by key monoclonal antibodies
This website provides interactive visualizations and raw data about the effects of mutations to the F protein of RSV as measured by the experiments described in Simonich et al (2026). That study measured how mutations to F affect its ability to mediate pseudovirus entry into 293T-TIM1 cells, and its neutralization by key monoclonal antibodies.
Because the datasets are so rich, the interactive visualizations here can be a better way to explore the data than the static figures in the paper. All the visualizations and data can be accessed by clicking on the gray boxes above for each type of measurement, namely:
For a single CSV file with the numerical values of all the QC-ed mutation effects, see here.
See Simonich et al (2026) for full study details.
The experimental measurements were made using pseudovirus deep mutational scanning with lentiviral particles pseudotyped with RSV F. These pseudoviruses do not encode any viral proteins other than F, and so are only able to undergo a single round of cell entry and are not fully replicative agents capable of spreading or causing disease. They therefore provide a safe way to study mutations to the F protein at biosafety-level-2 outside the context of actual pathogenic RSV.
Links to the deep mutational scanning measurements in numerical form (eg, as CSVs) are included on each of the main pages above (eg, the cell entry page has links to the CSV files with the raw data on how mutations affect cell entry).
For more detailed documentation of the analysis (including additional interactive plots and links to CSVs) as automatically generated by dms-vep-pipeline-3, see the Appendix.
The full computer code, processed data, and analysis pipeline is available on GitHub at https://github.com/dms-vep/RSV_Long_F_DMS.