Antibody Escape

We determined the effects of receptor binding protein mutations on antibody neutralization. Antibody selections were performed by incubating pseudovirus libraries with different concentrations of antibody, followed by infection of CHO cells expressing bat ephrin-B3. Neutralization curves were fit on the DMS data with polyclonal.

Schematic of antibody selections.

In total, we measured the effects of receptor binding protein mutations for six different antibodies that target three different epitopes.

Neutralization of unmutated Nipah RBP/F pseudovirus by different anti-RBP antibodies.

Location on RBP where antibodies bind (if structures are known).

Individual Antibody Selections

Individual antibody selection model fitting notebooks

Average Antibody Escape

Averaging antibody escape across libraries and replicate selections.

Average antibody escape notebooks

Antibody Escape Comprehensive Heatmaps

Additional control over filtering parameters. Users can adjust different parameters to filter the heatmap data. These provide more information and control compared to the final filtered heatmaps provided on the heatmaps page.

Antibody Escape Validations

To validate our deep mutational scanning measurements, we generated pseudoviruses expressing different receptor binding protein mutations. We tested whether neutralization by the antibody nAH1.3 correlated with our deep mutational scanning data.

Antibody validation notebook

To validate the escape measurements from DMS, we generated single RBP mutant pseudoviruses and tested their neutralization by antibody nAH1.3.